AI-Blue-Carba: A Rapid and Improved Carbapenemase Producer Detection Assay Using Blue-Carba With Deep Learning
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چکیده
منابع مشابه
Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers.
Performances of the Rapidec Carba NP test (bioMérieux) were evaluated for detection of all types of carbapenemases in Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa. In less than 2 h after sample preparation, it showed a sensitivity and specificity of 96%. This ready-to-use test is well adapted to the daily need for detection of carbapenemase producers in any laboratory...
متن کاملDetection of Carbapenemase Production in a Collection of Enterobacteriaceae with Characterized Resistance Mechanisms from Clinical and Environmental Origins by Use of Both Carba NP and Blue-Carba Tests.
Rapid-screening methods to confirm the presence of resistance mechanisms in multidrug-resistant bacteria are currently recommended. Carba NP and Blue-Carba tests were evaluated in carbapenemase-producing Enterobacteriaceae from hospital (n = 102) and environmental (n = 57) origins for detecting the different molecular classes among them. Both methods showed to be fast and cost-effective, with h...
متن کاملComparison of the Carba NP, Modified Carba NP, and Updated Rosco Neo-Rapid Carb Kit Tests for Carbapenemase Detection.
The accurate detection of carbapenemase-producing organisms is a major challenge for clinical laboratories. The Carba NP test is highly accurate but inconvenient, as it requires frequent preparation of fresh imipenem solution. The current study was designed to compare the Carba NP test to two alternative tests for accuracy and convenience. These were a modified Carba NP test that utilized intra...
متن کاملEvaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes
Background and purpose: Recently, increased resistance to carbapenem antibiotics among Pseudomonas aeruginosa has raised concerns. The Carba NP test is used for detection of carbapenemase types. This study aimed at applying Carba NP method for rapid detection of P. aeruginosa isolates producing carbapenemase enzymes. Materials and methods: A total of 97 clinical specimens was collected from pa...
متن کاملEvaluation of the Blue-Carba test for rapid detection of carbapenemases in gram-negative bacilli.
The Blue-Carba test (BCT) is a biochemical test for rapid ( 2 h) detection of carbapenemase production in Gram-negative bacilli directly from bacterial culture (1). It is based on the in vitro hydrolysis of imipenem by bacterial colonies (direct inoculation without prior lysis), which is detected by changes in pH values revealed by the indicator bromothymol blue (blue to green/yellow or green t...
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2020
ISSN: 1664-302X
DOI: 10.3389/fmicb.2020.585417